Salve a tutti!!qualkuno potrebbe kiarirmi il compito della biotina nella OLA(olinucleotide ligation assay)oppure indicarmi materiale da cui attingere info al riguardo?? grazie mille!!
Ti potrebbe servire? The oligonucleotide ligation assay (OLA) provides another method for detecting well-defined alleles that differ by a single base. OLA uses a pair of oligonucleotide probes (oligomers) that hybridize to adjacent segments of DNA including the variable base. The oligomer on the 5' or left-hand side of the pair is an allele-specific oligonucleotide (ASO) to one allele of the target. The last base at the 3' end of this ASO is positioned at the site of the target DNA's polymorphism; the ASO also has a biotin molecule at its 5' end that functions as a chemical hook. The oligomer on the 3' or right-hand side of the pair is the common oligomer (sequence is the same for the two different alleles.) The common oligomer is positioned at an invariable site next to the target DNA's polymorphism and is fluorescently labeled at its 3' end.